Green Mussel (Perna viridis.) 2nd Week of OJT

Wednesday, August 29th 2018. Today we were learned about Green Mussel (Perna viridis.) actually, everywhere we can found mussel in Philippine,  then we met Mr. Josel and Ms. Dona (our mentors), firstly they introduced us about green mussel and had some tour at the hatchery. Target of UPV’s Green Mussel Hatchery is to product seeds, for growth up culture. In UPV’s Green Mussel Hatchery also they have 4 Major :

1. Spawning 
2. Larvae culture
3. Special stage/ transition stage (more complicated)
4. Nursery stage

Mussel foods :

1. Isochrysis galbana
2. Chaetoceros sp.
3. Tetraselmis sp. 

For early stage we can use Chaetoceros sp. And Isochrysis galbana. 

Mussel -> have to provide them with phytoplankton (micro algae) because zooplankton more bigger than a mussel.

Spawning -> the sperm got in mild nature

Larvae leading -> takes (15 days +/-) net ideal for become a substrat.

water quality control for green mussel :

salinity : 30 ppt
temperature : 28 Celcius degree - 30  Celcius degree (but, in the spawning stage we need 30  Celcius degree.)

then, after he  gave a lesson about green mussel we identify which the bad larvae and the good larvae. the good one are ther is some spottings in their body and ther is no empty space in their body, but the bad one only shell, thers no spotting.

After identify the larvae then we had our lunch in canteen, pray, and go back to hatchery.

We also count the algae which are Isochrysis galbana, Chaetoceros sp., Tetraselmis sp. We use hemocytometer for count them and here are the results :

• Chaetocerus sp. : 

A block : 503 cells

B block : 381 cells

C block : 387 cells

D block : 421 cells

Corner Block=(503+381+387+421)/4 〖 × 10〗^4

= 4.320.000 cells/ml

• Tetraselmis sp. :

A block : 18 cells

B block : 21 cells

C block : 21 cells

D block : 21 cells

Corner Block=(18+21+21+21)/4 〖 × 10〗^4

= 202.500 cells/ml

• Isochrysis galbana:

A block : 662 cells

B block : 711 cells

C block : 692 cells

D block : 647 cells

Corner Block=(662+711+692+647)/4 〖 × 10〗^4

= 6.780.000 cells/ml

Then after we had this results we could synchronized  how much microalgae that we will gave to the culture of green mussel, for example :

Literature from how many algae are consumed by green mussel’s age (Mr. Josel, 2018) :

Day 1 – 4 old = 2.000 cells/ larvae/ day

Day 5 – 9 old = 5.000 cells/ larvae/ day

Day 10 – 15 old = 10.000 – 12.000 cells/ larvae/ day

V=((number of larvae)(feeding rate))/((algae cell concentration))

If we had 4.500.000 larvae, with age 6 days old, then we feed the mussel using Isochrysis galbana. Will be :

V=((4.500.000)(5000))/((6.780.000 cells/ml))

= 3.3 L/2 times feeding

= 1.65 L

At 03.30 pm we feed the mussel, then back to our boarding room. So tonight while writing this report I got a visitors, which are my bestie here, Stephen (pen pen) he one of my favorite person that Ive found in Miag ao. Pen pen come to my boarding house with his tita (aunti), his cousin, and his friends, they are very kind, also pen pen’s tita gave me a snack, im so blassed. And I was took a picture with pen pen’s tita. Here.